Dynamic Hydrogels for Biophysical Regulation of Cell Activities
Hydrogels with dynamic properties originated from reversible crosslinks can enable efficient cell adaptation of the matrix and support associated cellular functions. For instance, the spreading of encapsulted stem cells in such 3D hydrogels can be significantly accelerated.
Injectable Hydrogel Adhesives for Tissue Repair
Injectable hydrogel adhesives, especially those that can strongly adhere to tissues and feature near-native tissue mechanical properties, are desirable biomaterials for tissue repair. Compared to nonadhesive injectable hydrogels for minimally invasive delivery of therapeutic agents, they can better retain the delivered agents at targeted tissue locations and provide additional local physical barriers.
Microfluidics for Cardiavascular Tissue Engineering
According to the latest statistics report published in 2020, cardiovascular disease (CVD) caused around 43% of all deaths (4.1 million) across ESC (European Society of Cardiology) member countries in 2017, where more than 108.7 million people were living with CVD. We are currently developing microfluidic systems for cardiavascular tissue engineering.
2018-2020 [225 kCHF]
Supramolecular polymer self-assembly
Protein-polymer conjugates can be assembled by dual molecular recognitions. The third supramolecular interaction between alfa-CD and PEG triggers the formation of pseudo-polyrotaxanes (PPR) microcrystal structures that can cross-link the whole supramolecular polymeric system into hydrogel biomaterials. Such a bottom-up suramolecular self-assembly approach could avoid excessive chemical modification of natural and sythetic polymers, thereby providing a new strategy for developing novel biomaterials. This early study has also led to my postgraduate research projects, where supramolecular hydrogels are used for stem cell-based tissue engineering and regenerative medicine.
Supromolecular Hydrogel for Stem Cell Therapy and Tissue Regeneration
Biopolymer-based supramolecular hydrogels cross-linked by host–guest interactions are usually mechanically weak. In this project, we have develped a novel host–guest macromer (HGM) approach for mechanically robust supramolecular biopolymeric hydrogels, which have been tested in different tissue regeneration models.
Inspired by marine mussels, where different mussel foot proteins (Mfps) function cooperatively to achieve excellent wet adhesion, we herein report a dual-mode-mimicking strategy by modifying gelatin (Gel) biopolymers with a single-type thiourea–catechol (TU-Cat) functionality to mimic two types of Mfps and their mode of action. This strategy features a minor, yet impactful modification of biopolymers, which gives access to collective properties of an ideal injectable hydrogel adhesive.